Species Differentiation of Giardia by PCR
نویسندگان
چکیده
Molecular genetics, and in particular the development of the polymerase chain reaction (PCR), has provided a way to distinguish these organisms. One primer pair specific for the genus Giardia is available (Mahbubani et al. 1991) as is a primer pair specific for G.intestinalis, (Mahbubani et al. 1992). During experiments in this laboratory, designed to compare strains and species of Giardia using random amplification of polymorphic DNA (RAPD) (Williams et al. 1990 and Welsh et al. 1991), we detected a prominent amplified band with G.muris but not with G.intestinalis DNA. The base sequence of this band is reported here and using this information a primer pair was selected to amplify most of the sequence.
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